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The functional consequences of this hypothesis are discussed. For the deconvolution of the spectra, OriginPro 2017 b9.4.0.220 (OriginLab Corporation), a software peak analyzer tool (goal: Fit Peaks Pro baseline mode: constant Y 0 peak finding method: second derivative peak filtering: number of peaks 5 fitting function: Gaussian any parameters was not fixed) was used on all the spectra with adj. We hypothesize that some mitochondrial loop (tentatively loop M1) shows a high tendency to aggregate, being responsible for the observed features. From the difference spectra of the amide I, amide II, and amide A bands combined with dichroism spectra of the carboxyatractyloside-inhibited protein, we concluded that few structural differences exist between both states, affecting as few as 11 amino acids (3.5% of the protein) the structural changes consisted in the disappearance of large loop structure and the appearance of aggregated strands. To do so, the carboxyatractyloside-inhibited protein was used as a structural model for the protein in the CATR conformation and its spectrum was compared with that of the unliganded time-inactivated protein. developments in FTIR technique and its applications to protein secondary structure analysis and conformational study. First, the second derivative spectra were determined based on the ATR-FTIR spectra to determine the initial peak positions for curve fitting, and the peaks were fitted using a Gaussian function. It is important to note that second derivative analysis is often performed prior to deconvolution to clearly identify the peaks required for peak fitting. By Fourier transform infrared spectroscopy, we studied the structural changes involved in this denaturation process. Deconvolution of amide I region (16001700 cm 1) Protein secondary structure was analysed by means of curve fitting using MagicPlot 2.7.2. Peak deconvolution of the Amide I peak (Figure 4) of BSA was carried out using the OMNIC software.
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When solubilized in the detergent dodecyl maltoside the protein is in equilibrium between the so-called CATR and BA conformations and in a few hours it becomes nonfunctional, unable to bind either its inhibitors or its substrates. Therefore to analyse the output from both FTIR and GCMS, an analyst has to juggle between both instruments.
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The signal processing tool exclusively designed for FTIR is not applicable to GCMS chromatograms due to differences in data nature and characteristics. The ADP/ATP transporter shows a high instability when solubilized, making it difficult to obtain functional protein with sufficient purity for long-term spectroscopic studies. The software is typically instrumental and model dependent.